Golgi resident protein GCP60

ACBD3 (Golgi resident protein GCP60) is a 528-amino acid protein that plays a key role in maintaining Golgi architecture and regulating protein transport. It functions by interacting with giantin to stabilize Golgi structure and mediates trafficking between the endoplasmic reticulum and Golgi apparatus (UniProt: Q9H3P7). The protein also recruits and enhances the activity of PI4KB at the Golgi membrane, concentrating substrate availability for phosphorylation.

ACBD3 is localized to the Golgi apparatus, where it participates in steroid biosynthesis in testicular Leydig cells and supports intracellular membrane trafficking (UniProt: Q9H3P7). The protein has also been implicated in viral replication processes, functioning in aichi virus RNA synthesis through PI4KB recruitment.

In Alzheimer's disease, ACBD3 is upregulated in post-mortem AD brain tissue compared to age-matched controls, with a mean log2 fold-change of 0.29 (Chaparral AD proteomics). This elevation was detected across one subcellular fraction in a TMT-labeled quantitative proteomics study examining four distinct cellular compartments, suggesting that altered Golgi organization or membrane trafficking may be associated with pathological processes in AD.