NSFL1 cofactor p47

NSFL1C encodes NSFL1 cofactor p47, a 370-amino acid protein that modulates VCP (valosin-containing protein) ATPase activity and participates in multiple cellular processes including Golgi stack fragmentation and reassembly during mitosis, tER formation, and centrosomal regulation of Aurora A kinase (UniProt: Q9UNZ2). The protein functions as a regulatory cofactor within protein quality control and cytoskeletal organization pathways, with demonstrated roles in spindle orientation and mitotic progression.

In the context of Alzheimer's Disease, NSFL1C shows ambiguous regulation across subcellular fractions in post-mortem AD brain tissue compared to age-matched controls, with a mean log2 fold-change of 0.047 (Chaparral AD proteomics). This near-zero average change masks potentially divergent regulation across the four examined subcellular compartments (P2, P3, S2, S3), indicating that NSFL1C's involvement in AD pathology may be compartment-specific rather than showing consistent directional change across the proteome.